Methods for Optimizing Detection of Immunoassay Reactions Conducted within a Microfluidic Microplate

United States Patent Application 20180141046

Inventors: Aniruddha Puntambekar, Se Hwan Lee, Jungyoup Han, Chong H. Ahn, Winton Gibbons

A method for reading a microfluidic microplate having a plurality of cells is provided. The method includes determining target locations on the microfluidic microplate, each of the target locations being deviated from a center of each of the cells, and directing a beam centered at each of the target locations perpendicular to the microfluidic microplate, the beam having a predetermined diameter. Each of the plurality of cells includes a well structure including a side wall for a loading well, a through hole at a center of a base of the well structure, and a microfluidic channel formed in a spiral pattern configured to start from a first end of the microfluidic channel and end with a second end of the microfluidic channel at the base of the well structure, wherein the first end of the microfluidic channel is connected to the through hole, and the second end of the microfluidic channel includes an outlet hole.

Detection of Complexes of Tau and Amyloid

United States Patent Application 20130217147

Inventors: Winton Gibbons, Thomas Holzman, Lei Chang, Claude Lerner

The present technology relates generally to diagnostic and prognostic methods for neurological diseases such as mild cognitive impairment (MCI) and Alzheimer’s disease. In particular, the present disclosure relates to methods for detecting complexes of Tau, Tau variants, including phosphorylated variants, and amyloid containing molecules, as well as autoantibodies to those complexes or components of those complexes, in physiological fluid samples, which complexes are markers for neurological diseases such as mild cognitive impairment.

Assays for Clinical Assessments of Rheumatoid Arthritis

United States Patent Application 20110014632

Inventors: Winton Gibbons, Thomas Holzman, Claude Lerner

The disclosure provides methods of detecting autoantibodies present in the serum of subjects suffering from rheumatoid arthritis. The methods use capture probes and detection probes that can bind to the antifilaggrin autoantibodies or other epitope related autoantibodies. The presence, absence, and/or amount of the autoantibody complex may be detected, wherein the presence of the complex may indicate a positive diagnosis of rheumatoid arthritis.

Assays for Clinical Assessments of Disease-Associated Autoantibodies

United States Patent Application 20100144055

Inventors: Thomas Holzman, Winton Gibbons

The disclosure provides methods of detecting autoantibodies (AAs) in biological samples. The methods use capture probes that can bind to an disease-associated antigen/AA complex and a detection probe that can bind to the AAs. The presence, absence, and/or amount of the complex may be measured, wherein the presence of the complex may be diagnostic or prognostic of a disease or medical condition. The disclosure also provides methods of simultaneously detecting AAs and antigens in biological samples. The presence, absence, and/or amount of AAs and antigens may be measured, wherein the amount of antigen present and/or the amount of autoantibody present may be diagnostic or prognostic of a particular disease or medical condition.

Methods and Assays to Assess Cardiac Risk and Ischemia

United States Patent Application 20090233312

Inventors: Winton Gibbons, Thomas Holzman, Phillip Lefebvre, Gregory Shipp

The invention provides methods and apparatus to assess cardiac risk and ischemia by detecting or analyzing cardiac troponin levels. Also provided are methods to detect low levels of cardiac troponin in physiological fluid samples.